A5228
Sigma
Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse
clone 1A4, purified immunoglobulin
Description
| Immunogen | N-terminal synthetic decapeptide of α-smooth muscle actin. |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Specificity | The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | 1A4 (monoclonal) |
| mol wt | antigen mol wt ~42 kDa |
| species reactivity | rabbit, mouse, guinea pig, chicken, goat, sheep, snake, human, frog, rat, canine, bovine |
| application(s) | immunoblotting: suitable using smooth muscle cells |
| immunocytochemistry: suitable using smooth muscle cells | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 5-10 μg/mL using blood vessels in formalin-fixed, paraffin-embedded human tonsil or appendix tissue sections | |
| microarray: suitable | |
| isotype | IgG2a |
| Gene Information | human ... ACTA2(59) |
| rat ... Acta2(81633) | |
| mouse ... Acta2(11475) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Lowe, D.A., et al., Skeletal muscle-specific ablation of cyto-actin does not exacerbate the mdx phenotype. PLoS ONE 3, e2419, (2008) Abstract |
| Skalli, O., et al., A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103, 2787, (1986) |
